Isothermal Titration Calorimetry (ITC) Binding Services

BasePair Bio offers high-resolution Isothermal Titration Calorimetry (ITC) services to measure the thermodynamic properties of biomolecular interactions. ITC is a label-free, solution-based technique that provides direct measurements of binding affinity (Kd), enthalpy (ΔH), entropy (ΔS), and stoichiometry (n)—key parameters in drug discovery, biomolecular engineering, and aptamer selection.
Why Choose ITC?

  • Label-Free & Unbiased: Unlike fluorescence or SPR-based methods, ITC does not require molecular modifications.
  • Complete Thermodynamic Profile: ITC measures not only binding affinity but also enthalpic and entropic contributions, providing insights into molecular recognition.
  • True Solution-Phase Measurement: Unlike surface-based techniques, ITC analyzes interactions in their native environment without immobilization artifacts.
  • Broad Application Scope: Suitable for proteins, nucleic acids, small molecules, antibodies, aptamers, and more.

Our ITC Capabilities


At BasePair Bio, we use state-of-the-art microcalorimetry to support research across biotechnology, pharmaceuticals, and diagnostics. Our services include:

  1. Binding Affinity Determination

    • Measure dissociation constants (Kd) across a broad range (nanomolar to millimolar).
    • Optimize ligand-receptor interactions for drug discovery and aptamer development./li>

  2. Enthalpy and Entropy Profiling

    • Determine the driving forces of molecular binding (enthalpy- vs. entropy-driven interactions).
    • Provide insights for rational drug design and biomolecular optimization./li>

  3. Stoichiometry Analysis

    • Quantify the number of binding sites and interaction models.
    • Evaluate cooperative binding effects in multimeric proteins or nucleic acid complexes./li>

  4. Competitive Binding Assays

    • Evaluate ligand displacement in complex mixtures.
    • Determine relative affinities in multi-ligand systems./li>

  5. Custom Study Design and Data Analysis

    • Tailored experimental setup based on sample properties.
    • Expert analysis and interpretation to help you derive actionable insights.li>

Application

  • Aptamer Characterization – Assess aptamer-target interactions to guide selection and optimization.
  • Protein-Protein Interactions – Study enzyme-substrate, receptor-ligand, or antibody-antigen binding.
  • Small Molecule Drug Discovery – Screen potential drug candidates for target binding affinity.
  • Nucleic Acid Interactions – Analyze RNA/DNA binding events critical for gene regulation and therapeutics.
  • Enzyme Kinetics & Inhibition StudiesEnzyme Kinetics & Inhibition Studies

How Our Service Works

  1. Consultation & Study Design:Share your target molecules and study objectives.
  2. Sample Preparation:We provide detailed guidelines for buffer compatibility and sample conditions.
  3. ITC Measurement: High-precision experiments performed by our expert biophysicists.
  4. Data Analysis & Report:Deliverables include a comprehensive binding thermodynamics report, raw ITC traces, and expert interpretation.

Why Work With BasePair Bio?

  • Cutting-Edge ITC Instrumentation – Industry-leading microcalorimetry for ultra-sensitive measurements.
  • Expert Biophysical Analysis – Our team has deep expertise in molecular interactions and drug discovery.
  • Customizable Service Packages> – Tailored experiments to fit your project needs and budget.
  • Fast Turnaround and Confidentiality – Rapid project execution with strict data security protocols.

Get Started with Isothermal Titration Calorimetry (ITC) Binding Services
Interested in outsourcing your Isothermal Titration Calorimetry (ITC) Binding studies? Contact info@basepairbio.com today to discuss your project.